Itandukaniro mu ngamba zo kugenzura ubushyuhe bw'ibigega by'ubushyuhe bitandukanye mu mikorere y'ubushyuhe bw'ibinyabuzima

Intangiriro

Gushyushya ibikomoka ku binyabuzima ni inzira ihindura ibikoresho fatizo mo ibintu byibandwaho binyuze mu bikorwa bya mikorobe. Ikoreshwa cyane mu bijyanye n'imiti, ibiribwa, ubuhinzi, no kurengera ibidukikije. Mu gihe cyo gushyushya ibikomoka ku binyabuzima, ubushyuhe ni ikintu cy'ingenzi kigira ingaruka ku mikorere ya mikorobe, inzira za mikorobe, no kurema ibicuruzwa. Hari itandukaniro rinini mu ngamba zo kugenzura ubushyuhe hagati y'ibipimo bitandukanye by'ibigega byo gushyushya ibikomoka ku binyabuzima, ahanini bigaragarira mu mikorere myiza y'ubushyuhe, imiterere ya sisitemu yo kugenzura, umuvuduko w'ibisubizo, kugenzura ikoreshwa ry'ingufu, no gupima imikorere. Iyi ngingo izasesengura ku buryo burambuye itandukaniro mu ngamba zo kugenzura ubushyuhe za ibigega byo guhisha muri laboratwari, mu rwego rw'igerageza, no mu nganda, hamwe n'impamvu zabyo, hagamijwe gutanga ibitekerezo by'inyigisho zo kunoza no kongera uburyo bwo gukora imisemburo.

I. Uruhare rw'Ubushyuhe mu Ikorwa ry'Ubushyuhe

Ubushyuhe bugira ingaruka ku mikorere ya enzymes z'uturemangingo, uburyo uturemangingo twinjira mu mubiri, uburyo imikorere ya metabolic ihinduka, ndetse n'umuvuduko w'iremwa ry'ibikomoka ku bimera. Mu buryo bwinshi bwo guhisha, ubushyuhe bwiza ku turemangingo ni hagati ya dogere selisiyusi 20 na 40. Kurenga ubu bushyuhe bizagabanya ubushobozi bwo guhisha cyangwa se bikanatuma uturemangingo dupfa. Kubwibyo, kugumana ubushyuhe bukwiye kandi buhamye ni ngombwa kugira ngo imikorere ya fermentation irusheho kuba myiza kandi irusheho kuba nziza.

Sisitemu yo kugenzura ubushyuhe ntabwo ikeneye gusa kubungabunga ubushyuhe bwagenwe neza, ahubwo igomba no kugira ubushobozi bwo guhangana n’ibibazo nk’impinduka z’ubushyuhe zitunguranye (nk’ubwiyongere bw’udukoko butera ubushyuhe bwinshi) mu gihe cyo guhinga.

II. Uburyo bwo Kugenzura Ubushyuhe bw'Inyobo Zitandukanye zo Gushyushya

1. Igipimo cya Laboratwari (1L - 20L)

Udukoresho two gushyushya two ku rwego rwa laboratwari Ubusanzwe ni nto kandi zifite umuvuduko wo gukora ubushyuhe no gushonga vuba. Uburyo bwazo bwo kugenzura ubushyuhe bworoshye kandi muri rusange bugizwe n'ibi bikurikira:

• Sisitemu yo gushyushya: Ikoti rikoresha amashanyarazi rishyushya cyangwa inkoni ishyushya;
• Uburyo bwo gukonjesha: Amazi akonjeshwa anyura mu ikoti cyangwa mu byuma bikonjesha byubatswemo;
• Sisitemu yo kugenzura: Sisitemu yo kugenzura PID (Proportional-Integral-Derivative) ihujwe na thermistors kugira ngo igenzure ihindagurika hagati yo gushyushya no gukonjesha;
• Uburyo bwo gutanga amakuru ku bushyuhe: Thermocouples cyangwa thermistors bitanga ibimenyetso by'ubushyuhe mu gihe nyacyo.

Bitewe n'uko ari nto, ubushyuhe bukwirakwira ku buryo bworoshye, uburyo bwo kugenzura ubushyuhe bwihuta, kandi uburyo sisitemu ihagaze neza buri hejuru. Ihindagurika ry'ubushyuhe muri rusange riri hagati ya ± 0.2℃.

2. Igipimo cy'umupilote (50L - 500L)

Udukoresho two gushyushya ibintu mu buryo bwa pilot bikoreshwa mu kunoza imikorere no kugenzura uburyo bwo kuyikoresha. Ubuhanga bwa sisitemu buriyongera cyane:

• Uburyo bwo gushyushya: Uburyo bwo gutembera amazi ashyushye ukoresheje ikoti cyangwa ubushyuhe bw'umwuka
• Uburyo bwo gukonjesha: Ikoti rifite ibice bibiri rifite amazi akonjesha cyangwa imiyoboro yo gukonjesha imbere;
• Ibikoresho byo Kugenzura Ubushyuhe: Ubusanzwe bifite sisitemu yo kugenzura ya PLC, ishobora guhindura igipimo cy'amazi akonje n'ashyushye;
• Ahantu ho Gukurikirana: Ibikoresho byo kugenzura ubushyuhe bishobora gushyirwa ahantu henshi kugira ngo bigenzure itandukaniro ry'ubushyuhe mu bice bitandukanye by'amazi.

Muri iki cyiciro, ubushyuhe bukwirakwira ntabwo bukibaho kimwe. Cyane cyane mu buryo bwo gushyushya bufite ubukana bwinshi cyangwa ubucucike bwinshi bw’uturemangingo, ubushyuhe bukabije cyangwa ubukonje budahagije bushobora kubaho, bityo ni ngombwa kuziba icyuho binyuze mu kunoza imikorere yo gukangura no gusubiza neza uburyo bwo kugenzura.

3. Igipimo cy'inganda (1m³ kugeza kuri mirongo ya m³)

Ibigega byo gushyushya ibiribwa bikoreshwa mu nganda bahura n'ibibazo byinshi mu bijyanye no kugenzura ubushyuhe:

• Ikibazo cyo kohereza ubushyuhe neza: Uko ingano yiyongera, igipimo cy'ubuso n'ingano kiragabanuka, bigatuma ubushyuhe burushaho kugorana gushonga mu gipimo kimwe;
• Kwiyongera vuba k'ubushyuhe mu mubiri: Ubwinshi bw'uturemangingo n'imikorere yabyo mu mubiri bituma ubushyuhe bwiyongera vuba;
• Sisitemu yo gukonjesha irushijeho kuba nziza: Sisitemu nyinshi nka za jaketi zifite urwego rwinshi, ibyuma bikonjesha bikoresha imbaraga nyinshi, imiyoboro ikonjesha, n'iminara ikonjesha zirakoreshwa;
• Urwego rwo hejuru rw’imikorere ikora: Sisitemu za DCS (Sisitemu yo Kugenzura Ikwirakwizwa) cyangwa SCADA zikoreshwa mu gucunga ubushyuhe, bigatuma habaho kugenzura ubushyuhe bw’ahantu no guhindura imiterere y’ikirere;
• Ubwiyongere bw'ubushyuhe: Kwitwara gahoro, kugenzura ubushyuhe bitinze, bityo bigasaba guhanura no guhindura hakiri kare;

Byongeye kandi, mu gihe cyo gushushanya ibigega binini byo gushyushya, hakenewe icyerekezo cya CFD (Computational Fluid Dynamics) kugira ngo hamenyekane uko ubushyuhe bukwirakwira, hagakurikizwa imiterere y'imiyoboro ikonjesha n'imiterere y'ibitera imbaraga.

III. Isesengura ry'Itandukaniro mu Ngamba zo Kugenzura Ubushyuhe

1. Umuvuduko wo kugenzura ubushyuhe

Sisitemu yo mu rwego rwa laboratwari irakira vuba, irangiza guhindura ubushyuhe mu masegonda make;

Sisitemu y'igipimo cy'umupilote ifite umuvuduko mpuzandengo wo gusubiza, bifata amasegonda icumi kugeza ku minota myinshi;

Sisitemu yo mu rwego rw'inganda igira igisubizo gito cyane, gishobora gufata iminota myinshi cyangwa irenga. Kubwibyo, ingamba zo guhanura (guhanura) zikunze gukoreshwa.

Sisitemu ya MIKEBIO yo gushyushya ingano y'inganda

2. Uburyo butandukanye bwo kugenzura ubushyuhe

Ku buryo buciriritse, uburyo bw'ingenzi bwo gushyushya hakoreshejwe amashanyarazi no kugenzura amazi akonje ni bwo buryo;

Ku buryo bwo gupima no hejuru yabwo, ubushyuhe bw'umwuka no guhindura ikigereranyo cy'imvange y'amazi akonje ni byo bikunze gukoreshwa;

Ku bigega by’inganda, hakenewe uburyo bwinshi bwo kugenzura, kandi amasoko akonje/ashyushye ahita ahinduka bitewe n’ubushyuhe bw’ibidukikije, icyiciro cyo gushyushya, n’impinduka z’umutwaro w’ubushyuhe.

3. Uburyo ubushyuhe bukwirakwira mu buryo bumwe

Ibigega bito, bitewe n'uko ari bito, bishobora kugera ku bushyuhe bungana binyuze mu gukangura;

Ibigega by'umupilote bigomba kuvanga uburyo bwo kuvanga n'uburyo bwo kugenzura ubushyuhe;

Ibigega by'inganda bisaba imiterere y'imbere igoye cyane, nko gupima ubushyuhe bw'ingingo nyinshi, gukurura amazi mu cyerekezo, no gukonjesha mu byiciro, nibindi.

1. Ingorane mu miyoborere yo kugenzura ubushyuhe no kunoza ubushyuhe

IV. Isano iri hagati yo kugenzura ubushyuhe n'imikorere myiza y'ubushyuhe

Ubuziranenge bw'igenzura ry'ubushyuhe mu buryo butaziguye ingaruka igipimo cy'ubwiyongere bw'udukoko duto n'ubwoko bwa metabolitike ikorwa. Urugero, mu gushyushya imiti yica udukoko, kwiyongera k'ubushyuhe bishobora kwihutisha ubwiyongere bw'ibinyabuzima, ariko bikabuza ikorwa rya metabolitike y'inyongera. Ku bikorwa aho poroteyine ikoreshwa mu gushyushya ari poroteyine ifata ubushyuhe, nka sisitemu yo kugaragaza poroteyine, kunyura mu bushyuhe bishobora gutuma imiterere ya poroteyine ihindagurika nabi cyangwa ikorwa ry'imibiri ikubiyemo, bigabanura cyane umusaruro w'umusaruro.

Byongeye kandi, mu gihe cyo kongera ibikorwa, itandukaniro mu ngamba zo kugenzura ubushyuhe naryo ni imwe mu mpamvu zikomeye zituma amakuru yo muri laboratwari agorana gukopwa mu bihe by'inganda.

V. Umwanzuro

Kugenzura ubushyuhe ni ikintu cy'ingenzi mu gutunganya fermentation ya bioprocessing. Udukoresho tw’ubushyuhe dutandukanye dukenera ingamba zo kugenzura ubushyuhe. Gutunganya fermentation ntoya byibanda ku gusubiza vuba no guhagarara neza, hagati bisaba kuringaniza umuvuduko w’ibisubizo n’ikwirakwizwa ry’ubushyuhe rimwe, mu gihe mu nganda bisaba ikoranabuhanga rigezweho no gutegura gahunda. Hamwe n’iterambere ry’ikoranabuhanga ry’inganda n’isesengura ry’amakuru, sisitemu zo kugenzura ubushyuhe mu gihe kizaza zizaba zirushijeho kuba nziza kandi zinoze, zitanga inkunga ikomeye ku buryo bwo gutunganya fermentation bunoze kandi bugenzurwa.

Ku rundi ruhande, iyo uhisemo imashini itunganya bioreactor, ni ngombwa gushaka uruganda rusanzwe rufite izina ryiza kandi rukomeye, kugira ngo rurebe neza ireme ry'ibicuruzwa. Jiangsu Mike Biotechnology Co., Ltd. yashinzwe mu 2008, yibanda ku ibikoresho byo gushyushya ibikomoka ku binyabuzima Ubushakashatsi n'iterambere, umusaruro n'ubucuruzi by'ibigo bikoresha ikoranabuhanga rihanitse. Isosiyete yacu ifite urubuga rwo gushyushya imashini mu buryo bw'icyitegererezo, ruhujwe n'ubuhanga n'ikoranabuhanga, kandi rwubatse uburyo bwo gushyushya imashini mu buryo bw'icyitegererezo bugezweho, bushobora kunoza uburyo bwo gushyushya imashini mu buryo bw'icyitegererezo, bushyiraho iterambere ry'uburyo bwose bwo gushyushya imashini mu buryo bw'icyitegererezo kuva ku gushyushya imashini mu buryo bw'icyitegererezo kugeza ku gutandukanya no gutunganya ibikoresho mu buryo bw'icyitegererezo, kandi bushyiraho ikigo cy'abaganga n'aho gukorera ubushakashatsi bugezweho.

Isosiyete yacu ifite uruganda rukora ibikoresho bisanzwe, rufite ibikoresho n'ibikoresho byuzuye bikenewe kugira ngo ishyirweho, harimo ibikoresho bitandukanye by'imashini, imashini ikoresha arc suitting, imashini itunganya imashini, imashini ikata n'imashini ipima amakosa hamwe n'ibindi bikoresho birenga 60 byo gukora no gupima, uburyo bwo gukora byose bugezweho, kugira ngo abakiriya babone ubuziranenge n'ubufasha mu bya tekiniki.