Umahluko kwiindlela zokulawula ubushushu kwiitanki zokubilisa ezinobukhulu obahlukeneyo kwiinkqubo zokubilisa zebhayoloji

Intshayelelo

Ukubiliswa kwezinto eziphilayo yinkqubo eguqula izinto eziluhlaza zibe ziimveliso ekujoliswe kuzo ngokusebenzisa imisebenzi ye-metabolism ye-microorganisms. Isetyenziswa kakhulu kwiindawo zamayeza, ukutya, ezolimo, kunye nokukhuselwa kwendalo. Ngexesha lenkqubo yokubiliswa, ubushushu buyiparameter ephambili echaphazela umsebenzi we-microbial, iindlela ze-metabolism, kunye nokwakheka kwemveliso. Kukho umahluko omkhulu kwizicwangciso zokulawula ubushushu phakathi kwezikali ezahlukeneyo zeetanki zokubiliswa, ezibonakala kakhulu ekusebenzeni kakuhle kobushushu, ulwakhiwo lwenkqubo yolawulo, isantya sempendulo, ulawulo lokusetyenziswa kwamandla, kunye nokwandiswa kwenkqubo. Eli nqaku liza kuhlalutya ngokucwangcisiweyo umahluko kwizicwangciso zokulawula ubushushu ze iitanki zokubilisa kwilebhu, kwi-pilot, nakwi-industrial scales, kunye nezizathu zazo, ngenjongo yokubonelela ngeengxelo zethiyori zokuphucula nokwandisa iinkqubo zokubilisa.

I. Indima yoLawulo loBushushu kwiNkqubo yokuBilisa

Ubushushu buchaphazela umsebenzi we-enzyme weeseli ze-microbial, ukuvuleka kwee-membranes zeseli, ukukhethwa kweendlela ze-metabolic, kunye nesantya sokwakheka kweemveliso. Kwiinkqubo ezininzi zokubila, uluhlu olufanelekileyo lobushushu kwii-microorganisms luphakathi kwama-20 nama-40 degrees Celsius. Ukudlula olu luhlu kuya kukhokelela ekunciphiseni ukusebenza kakuhle kwe-metabolic okanye nokufa kweeseli. Ke ngoko, ukugcina ubushushu obufanelekileyo nobuzinzileyo kuyimfuneko yokuqinisekisa ukusebenza kakuhle kwe-fermentation kunye nomgangatho wemveliso.

Inkqubo yokulawula ubushushu ayifuni nje ukugcina ubushushu obumiselweyo buzinzile kodwa kufuneka ikwazi ukuphendula ngokukhawuleza ukujongana nemingeni efana nokutshintsha kobushushu ngesiquphe (njengokwanda ngokukhawuleza kweentsholongwane ezivelisa ubushushu obukhulu be-metabolic) ngexesha lenkqubo yokubilisa.

II. Iinkqubo zoLawulo loBushushu beeNqwelo zokuBilisa eziNqanaba ezahlukeneyo

1. Isikali seLebhu (1L - 20L)

Iinqanawa zokubilisa ezikumgangatho welebhu Zihlala zincinci ngobukhulu kwaye zikhawuleza ukuvelisa ubushushu kunye nokusasazwa. Iinkqubo zazo zokulawula ubushushu zilula kwaye ngokubanzi ziquka ezi zinto zilandelayo:

• Inkqubo yokufudumeza: Ijakethi yokufudumeza yombane okanye intonga yokufudumeza;
• Inkqubo Yokupholisa: Ukujikeleza kwamanzi apholileyo ngejakethi okanye iikhoyili zokupholisa ezakhelwe ngaphakathi;
• Inkqubo yoLawulo: Inkqubo yokulawula ye-PID (Proportional-Integral-Derivative) eqhagamshelwe kwii-thermistors ukulawula ukutshintsha phakathi kokufudumeza nokupholisa;
• Impendulo yoBushushu: Ii-thermocouples okanye ii-thermistors zibonelela ngemiqondiso yobushushu ngexesha langempela.

Ngenxa yobukhulu bazo obuncinci, ubushushu busasazwa ngokulinganayo ngokulula, impendulo yolawulo lobushushu ikhawuleza, kwaye uzinzo lwenkqubo luphezulu. Ukuguquguquka kobushushu ngokubanzi kungaphakathi kwe-±0.2℃.

2. Isikali soMqhubi (50L - 500L)

Iinqanawa zokubilisa ezisetyenziswa ngokulinganisa umqhubi zisetyenziselwa ukuphucula inkqubo kunye nokuqinisekisa ukwanda. Ubunzima benkqubo buyanda kakhulu:

• Indlela yokufudumeza: Inkqubo yokujikeleza amanzi ashushu ngejakethi okanye ngokufudumeza ngomphunga
• Indlela Yokupholisa: Ijakethi enamaleko amabini enokujikeleza kwamanzi okupholisa okanye iikhoyili zokupholisa zangaphakathi;
• Izixhobo zoLawulo loBushushu: Zihlala zixhotyiswe ngenkqubo yolawulo ye-PLC, enokulungisa umlinganiselo wamanzi abandayo/ashushu;
• Iindawo Zokubeka Iliso: Izinzwa zobushushu zingabekwa kwiindawo ezininzi ukujonga umahluko wobushushu kwiindawo ezahlukeneyo zolwelo.

Kule nqanaba, ukusasazwa kobushushu akusafani ngokupheleleyo. Ingakumbi kwiinkqubo zokubilisa ezinobunzima obuphezulu okanye ezinoxinano olukhulu lweeseli, ubushushu obuphezulu okanye ukupholisa ngaphantsi kobushushu kusenokubakho, ngoko ke kuyimfuneko ukuhlawula ngokuphucula ukusebenza kakuhle kokuxukuxa kunye nokukwazi ukuphendula kwenkqubo yolawulo.

3. Isikali seShishini (1m³ ukuya kumashumi aliqela ee-m³)

Iitanki zokubilisa ezikwizinga lemizi-mveliso bajongene nemingeni engakumbi kulawulo lobushushu:

• Ingxaki yokusebenza kakuhle kokudluliselwa kobushushu: Njengoko ivolumu isanda, umlinganiselo womphezulu nomthamo uyancipha, nto leyo eyenza kube nzima ngakumbi ukuba ubushushu buphele ngaphakathi kwevolumu yeyunithi;
• Ukuqokelelana ngokukhawuleza kobushushu be-metabolism: Uxinzelelo olukhulu lweeseli kunye ne-metabolism esebenzayo kukhokelela ekuqokeleleni ngokukhawuleza kobushushu;
• Inkqubo yokupholisa ephuculweyo: Iinkqubo ezininzi ezifana neejakethi ezineeleya ezininzi, ii-chillers ezinamandla aphezulu, ii-cooling coils, kunye nee-cooling towers ziyasetyenziswa;
• Inqanaba eliphezulu lokuzenzekela: Iinkqubo ze-DCS (Distributed Control System) okanye ze-SCADA zisetyenziselwa ulawulo lobushushu, zivumela ulawulo lobushushu bezoni kunye nohlengahlengiso oluguquguqukayo;
• Ukwanda kwe-innertia yobushushu: Ukusabela kancinci, ulawulo lobushushu olulibazisekileyo, ngaloo ndlela kufuna ukuxelwa kwangaphambili kunye nokulungiswa;

Ukongeza, ngexesha lokuyilwa kweetanki ezinkulu zokubilisa, kufuneka ukulinganisa i-CFD (Computational Fluid Dynamics) ukuze kuqikelelwe ukusasazwa kwentsimi yobushushu, kukhokelwa uyilo lwemibhobho yokupholisa kunye noyilo lwee-agitators.

III. Uhlalutyo lomahluko kwiindlela zokulawula ubushushu

1. Isantya sokuPhendula soLawulo loBushushu

Inkqubo yesikali selabhoratri iphendula ngokukhawuleza, igqibezela ukulungiswa kobushushu kwimizuzwana embalwa;

Inkqubo yesikali sovavanyo inesantya esiphakathi sokuphendula, ithatha amashumi emizuzwana ukuya kwimizuzu embalwa;

Inkqubo yomlinganiselo wemizi-mveliso inempendulo ecothayo, enokuthabatha imizuzu eliqela okanye nangaphezulu. Ke ngoko, icebo lokuqikelela (ulawulo lokuqikelela) lidla ngokusetyenziswa.

Inkqubo yokubilisa isikali semizi-mveliso ye-MIKEBIO

2. Ukwahlukahluka kweendlela zokulawula ubushushu

Kwiinkqubo ezincinci, ukufudumeza ngombane kunye nolawulo lwamanzi abandayo zezona ndlela ziphambili;

Kwiinkqubo zesikali somqhubi kunye nezingaphezulu, ukufudumeza ngomphunga kunye nokulungiswa komlinganiselo womxube wamanzi okupholisa kudla ngokusetyenziswa;

Kwiitanki ezisetyenziswa kwimizi-mveliso, kufuneka ulawulo lweendlela ezininzi, kwaye imithombo ebandayo/eshushu itshintshwa ngokuzenzekelayo ngokusekelwe kubushushu bendalo, inqanaba lokubila, kunye notshintsho kumthwalo wobushushu.

3. Ukufana koSasazo loBushushu

Amatanki amancinci, ngenxa yobukhulu bawo obuncinci, anokufikelela kubushushu obufanayo ngokuxukuxa;

Iitanki zesikali sepilot kufuneka zidibanise ukuxuba kunye nenkqubo yokulawula ubushushu;

Iitanki zoshishino zifuna uyilo oluntsonkothileyo lwesakhiwo sangaphakathi, njengokulinganisa ubushushu obuneendawo ezininzi, ukuhamba komoya oshukumisayo, kunye nokupholisa okuneeleya, njl.

1. Ubunzima kwiMigqaliselo yoLawulo loBushushu kunye nokuLungiselela

IV. Ubudlelwane phakathi koLawulo loBushushu kunye nokusebenza kakuhle kokuBilisa

Ukuchaneka kolawulo lobushushu ngokuthe ngqo ichaphazela izinga lokukhula kwee-microorganisms kunye nohlobo lwee-metabolites eziveliswayo. Umzekelo, ekuvuthweni kwee-antibiotic, ukunyuka kobushushu kunokukhawulezisa ukuqokelelwa kokuqala kwe-biomass, kodwa kuthintele ukwakheka kwee-metabolites zesibini. Kwiinkqubo apho imveliso yokuvutshelwa iyiproteni ebuthathaka kubushushu, njengeenkqubo zokubonakalisa iiproteni eziphinda-phindayo, ukuphambuka kubushushu kunokukhokelela ekusongweni okungalunganga kwesakhiwo seproteni okanye ukwakheka kwemizimba yokubandakanywa, okunciphisa kakhulu isivuno semveliso.

Ngaphezu koko, ngexesha lokwandiswa kwenkqubo, umahluko kwizicwangciso zokulawula ubushushu uphakathi kwezona zizathu ziphambili zokuba kutheni kunzima ukukopisha idatha yelabhoratri phantsi kweemeko zoshishino.

V. Isiphelo

Ulawulo lobushushu luphawu oluphambili kwi-bioprocessing fermentation. Iinqanawa zokubilisa ezinobukhulu obahlukeneyo zifuna amaqhinga okulawula ubushushu ajoliswe kuwo. Ukubilisa okuncinci kugxile ekuphenduleni ngokukhawuleza nasekuzinzeni, okuphakathi kufuna ulungelelwaniso phakathi kwesantya sempendulo kunye nokusasazwa kobushushu obufanayo, ngelixa okukwimizi-mveliso kufuna ukuzenzekela okuphezulu kunye nocwangciso olucwangcisiweyo. Ngophuhliso lwetekhnoloji yokuvelisa ekrelekrele kunye nohlalutyo lwedatha, iinkqubo zokulawula ubushushu zexesha elizayo ziya kuba nobukrelekrele ngakumbi kwaye zichaneke ngakumbi, zibonelela ngenkxaso enamandla kwiinkqubo zokubilisa ezisebenzayo nezilawulekayo.

Kwelinye icala, xa ukhetha i-bioreactor fermenter, kuyimfuneko ukufumana umenzi oqhelekileyo onegama elihle kunye namandla, ukuze kuqinisekiswe umgangatho wemveliso. I-Jiangsu Mike Biotechnology Co., Ltd. yasungulwa ngo-2008, igxile kakhulu izixhobo zokubilisa eziphilayo uphando nophuhliso, imveliso kunye nokuthengiswa kwamashishini asebenza ngobuchwepheshe obuphezulu. Inkampani yethu ineqonga lokuvubela elijikelezayo, elidityaniswe nobukrelekrele kunye noomatshini, kwaye yakhe inkqubo yokuvubela evuthiweyo, enokwenza ukuba inkqubo yokuvubela elijikelezayo iphumelele, iqalise uphuhliso lwazo zonke iinkqubo zenkqubo ukusuka ekuvelisweni kokuvubela elijikelezayo ukuya ekuhlukanisweni nasekucocweni kwemveliso yokuvubela, kwaye iseke indawo yokusebenza yoogqirha kunye nelebhu yanamhlanje.

Inkampani yethu inesityalo semveliso esisemgangathweni, esinezixhobo ezipheleleyo nezixhobo ezifunekayo ukuze kufakelwe, kuquka izixhobo ezahlukeneyo zoomatshini, umatshini wokuwelda we-argon arc ozenzekelayo, umatshini wokupolisha ozenzekelayo, umatshini wokusika ozenzekelayo kunye nomatshini wokufumanisa iziphene kunye nezinye izixhobo ezingaphezu kwama-60 zemveliso kunye novavanyo, inkqubo yemveliso yonke ifezekisa ukusebenza okusemgangathweni kwanamhlanje, ukubonelela abathengi ngomgangatho ophezulu wobungcali kunye nenkxaso yobugcisa.